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basic fibroblast growth factor bfgf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec basic fibroblast growth factor bfgf
    Basic Fibroblast Growth Factor Bfgf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bfgf/Human+FGF-2%2C+premium+grade/pm42336896-211-24-29
    Average 95 stars, based on 147 article reviews
    basic fibroblast growth factor bfgf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas
    Article Snippet: .. GSCs were cultured as 3D spheres in serum-free DMEM-F12 medium (Lonza) containing 1 x BIT100 (Provitro), 2 mM L-Glutamine, 30 U/ml Pen-Strep, 1 U/ml Heparin (Sigma), 20 ng/ml bFGF (Miltenyi, 130-093-841) and 20 ng/ml EGF (Provitro, 1325950500) (NCH601, NCH421k, NCH644) or serum-free Neurobasal medium (Thermo Fisher, Cat# 21103049) supplemented with 1X B-27TM Supplement (Thermo Fisher, Cat# 17504044), 2 mM UltraGlutamine (LONZA, Cat#BE17-605E/U1), 0,1 U/mL heparin (Sigma-Aldrich, Cat# H3149), 20 ng/mL bFGF (Miltenyi, Cat# 130-093-839), 20 ng/mL EGF (Provitro, Cat# 1325960500) and 100 U/mL penicillin‒streptomycin (remaining GSCs). .. PDOX tumors were dissociated using the MACS Neural Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions.

    Article Title: Modeling common Alzheimer's disease with high and low polygenic risk in human iPSC: A large-scale research resource.
    Article Snippet: Vector transduced cells were transferred onto mitotically inactivated CF1 Mouse Embryonic Feeder cells (Millipore, PMEF-CFL-C) on 0.1% gelatin (Sigma, G1393)-coated plates. .. From day 3, cells were cultured in Knock-Out serum replacement medium (Knock-out DMEM [Gibco, 10829-018], 20% KO serum replacement [Gibco, 10828-028], 2 mM Glutamax [Gibco, 35030-1], 1% non-essential amino acids [Gibco, 11140-035], 100 units/ mL penicillin and 100 μg/mL streptomycin [Gibco, 15140- 122], 55 μM 2-mercaptoethanol [Gibco, 31350-010], and 5 ng/mL bFGF [Miltenyi Biotec, 130-093-842]). ..

    Article Title: FGFR1 drives metabolic adaptation associated with temozolomide resistance in glioblastoma.
    Article Snippet: Therapy resistance is a major limitation in therapeutic efficacy for glioblastoma (GBM) patients, positioning GBM among the deadliest tumor types.. In this work, we have dissected resistance mechanisms in GBM, which resulted in the identification of FGFR1 pathway as a major controller of the signaling and metabolic rewiring associated to temozolomide (TMZ) resistance.. Hence, in FGFR1-positive, p53 WT GBM cells, FGFR1 controls a p53-mediated cell cycle arrest to allow DNA repair in response to TMZ.

    Article Title: Modeling common Alzheimer’s disease with high and low polygenic risk in human iPSC: A large-scale research resource
    Article Snippet: Vector transduced cells were transferred onto mitotically inactivated CF1 Mouse Embryonic Feeder cells (Millipore, PMEF-CFL-C) on 0.1% gelatin (Sigma, G1393)-coated plates. .. From day 3, cells were cultured in Knock-Out serum replacement medium (Knock-out DMEM [Gibco, 10829-018], 20% KO serum replacement [Gibco, 10828-028], 2 mM Glutamax [Gibco, 35030-1], 1% non-essential amino acids [Gibco, 11140-035], 100 units/mL penicillin and 100 μg/mL streptomycin [Gibco, 15140-122], 55 μM 2-mercaptoethanol [Gibco, 31350-010], and 5 ng/mL bFGF [Miltenyi Biotec, 130-093-842]). ..

    Knock-Out:

    Article Title: Modeling common Alzheimer's disease with high and low polygenic risk in human iPSC: A large-scale research resource.
    Article Snippet: Vector transduced cells were transferred onto mitotically inactivated CF1 Mouse Embryonic Feeder cells (Millipore, PMEF-CFL-C) on 0.1% gelatin (Sigma, G1393)-coated plates. .. From day 3, cells were cultured in Knock-Out serum replacement medium (Knock-out DMEM [Gibco, 10829-018], 20% KO serum replacement [Gibco, 10828-028], 2 mM Glutamax [Gibco, 35030-1], 1% non-essential amino acids [Gibco, 11140-035], 100 units/ mL penicillin and 100 μg/mL streptomycin [Gibco, 15140- 122], 55 μM 2-mercaptoethanol [Gibco, 31350-010], and 5 ng/mL bFGF [Miltenyi Biotec, 130-093-842]). ..

    Article Title: Modeling common Alzheimer’s disease with high and low polygenic risk in human iPSC: A large-scale research resource
    Article Snippet: Vector transduced cells were transferred onto mitotically inactivated CF1 Mouse Embryonic Feeder cells (Millipore, PMEF-CFL-C) on 0.1% gelatin (Sigma, G1393)-coated plates. .. From day 3, cells were cultured in Knock-Out serum replacement medium (Knock-out DMEM [Gibco, 10829-018], 20% KO serum replacement [Gibco, 10828-028], 2 mM Glutamax [Gibco, 35030-1], 1% non-essential amino acids [Gibco, 11140-035], 100 units/mL penicillin and 100 μg/mL streptomycin [Gibco, 15140-122], 55 μM 2-mercaptoethanol [Gibco, 31350-010], and 5 ng/mL bFGF [Miltenyi Biotec, 130-093-842]). ..

    Clinical Proteomics:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19
    Article Snippet: .. HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml −1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml −1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Recombinant:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19
    Article Snippet: .. HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml −1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml −1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    other:

    Article Title: Ubiquitous MEIS transcription factors actuate lineage-specific transcription to establish cell fate
    Article Snippet: bFGF , Miltenyi Biotech , 130-115-009.



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